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KMID : 0613820040140020255
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Journal of Life Science
2004 Volume.14 No. 2 p.255 ~ p.262
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Application of DNA Content and Total Protein Concentration to Predict Blooms Caused by Cochlodinium polykrikoides (Dinophyceae) in Korean Coastal Waters
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Cho Eun-Seob
Choi Yong-Kyu
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Abstract
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We applied nuclear DNA content stained with 4’-6’-diamidino-2-phenylindole (DAPI) and total protein concentration to predict the existence of Cochlodinium polykrikoides before huge blooms occurred, based on a short-term survey at sites in the South Sea. Fluctuations in environmental conditions and nutrients (nitrate, nitrite, and phosphate) were of a similar range, regardless of sampling sites or early and middle field observations. However, C. polykrikoides abundance was significantly different depending on the station, with a higher cell density of 34, 62, and 57 cells L-1 at Stn C2, C5, and C6, respectively than what was found in early August, 2000. In mid August, 2000, the highest cell density of 547 cells L-1 at Stn C3 was observed. The relationship between C. polykrikoides abundance, DAPI-stained DNA content, and total protein concentration was a positive correlation coefficient, in particular a higher positive correlation was exposed to even a smaller abundance of C. polykrikoides. These results suggest that DNA stained by DAPI and total protein concentration could play an important index in easily predicting the presence of C. polykrikoides before blooms.
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KEYWORD
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Cochlodinium polykrikoides, image analysis system, nuclear DNA content, prediction, total protein concentration, red tide
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